Effects of Tramadol on Histopathological and Biochemical Parameters in Mice (Mus musculus) Model

The present study was conducted to determine the histopathological and biochemical changes in liver due to injection of tramadol in albino mice (Mus musculus). For this purpose, forty albino mice (25-30g) were divided into four groups, each group carried ten mice (three experimental and control group). Experimental groups (B, C and D) were injected tramadol intramuscularly equal to 12.5 mg, 25mg and 50 mg/kg body weight/day respectively for fourteen days. Biochemical analysis indicated that the levels of serum aminotransferase (ALT, AST) significantly (P < 0.05) increased than the control group. Similarly creatinine and blood urea nitrogen (BUN) were also increased significantly (P <0.05) in the experimental groups than control. The histopathological studies indicated the necrosis, vacuolization, central vein dilation, hemorrhage, cytolysis and complete cell membrane degeneration in hepatocytes in the treated groups. Therefore it is recommended that tramadol should be taken only with the prescription of doctor and self medication of this medicine may be hazardous.